Journal: NPJ Breast Cancer

Article Title: NDRG4 promoter hypermethylation is a mechanistic biomarker associated with metastatic progression in breast cancer patients

doi: 10.1038/s41523-019-0106-x

Figure Lengend Snippet: NDRG4 depletion in breast tumor cells increases lymph node adhesion and cell migration toward VN. a NDRG4 expression meta-analysis in human breast cancer cell lines using GOBO application . Breast cancer subtypes can be identified by different colors: red = Basal A, gray = Basal B and blue = Luminal cells. b Analysis of NDRG4 mRNA expression levels in MCF-7 cells transfected with NDRG4-shRNAs or scramble control (shSCR). Expression levels are relative to wild type cells and normalized to hydroxymethylbilane synthase (HMBS) gene. Error bars represent SEM of biological replicates ( n = 5). *** p < 0.001, ns = not significant, by one-way ANOVA. c Western blot analysis of NDRG4 expression in cytoplasmic extracts of MCF-7 shNDRG4s or shSCRs cells, using antibodies against NDRG4 and Actin. MCF-7 cells express all the three isoforms of NDRG4: 37 kDa (NDRG4-B), 39 kDa (NDRG4-B var ) and 41 kDa isoform (NDRG4-H). For simplicity, results obtained for two independent shNDRG4 clones were grouped and presented as the shNDRG4 group. ( d , left) Representative images of adherent red fluorescent MCF-7 cells on frozen rat lymph node sections. d , e Quantification of adherent MCF7 ( d ) and T47D ( e ) breast tumor cells in lymph nodes sections (right, n = 4 independent experiments for MCF-7 and 3 for T47D, ** p < 0.01 by two-tailed t test). f Stationary adhesion assays showed that NDRG4 knockdown promotes ‘adhesive switch’ between FN and VN. Error bars represent SEM of biological replicates ( n = 4). Cells do not adhere to albumin control (BSA, data not shown). * p < 0.01, ** p < 0.01, ns = not significant by two-tailed t tests. g , h Haptotactic cell migration toward VN was analyzed by transwell migration assay in MCF-7 ( g ) and T47D ( h ) cell lines. Error bars represent SEM of biological replicates ( n = 4). *** p < 0.001 by two-tailed t tests

Article Snippet: For stable transfection of NDRG4 shRNA into MCF-7 and T47D breast tumor cells, the pGFP-V-RS vector containing shRNA inserts which targeted the 3′-unttranslated region of NDRG4 (TRCN0000134583 and TRCN0000137216) was purchased (Origene).

Techniques: Migration, Expressing, Transfection, Western Blot, Clone Assay, Two Tailed Test, Transwell Migration Assay

Journal: NPJ Breast Cancer

Article Title: NDRG4 promoter hypermethylation is a mechanistic biomarker associated with metastatic progression in breast cancer patients

doi: 10.1038/s41523-019-0106-x

Figure Lengend Snippet: NDRG4 knockdown promotes clustering of β1-integrin at the leading edge of T47D cells. Representative confocal images of β1 integrin subunit (MAB1965, green) at the ventral cell surface of VN-adherent T47D shNDRG4 or shSCR cells

Article Snippet: For stable transfection of NDRG4 shRNA into MCF-7 and T47D breast tumor cells, the pGFP-V-RS vector containing shRNA inserts which targeted the 3′-unttranslated region of NDRG4 (TRCN0000134583 and TRCN0000137216) was purchased (Origene).

Techniques:

Journal: BioMed Research International

Article Title: Patched Targeting Peptides for Imaging and Treatment of Hedgehog Positive Breast Tumors

doi: 10.1155/2014/525680

Figure Lengend Snippet: In vitro uptake of 99m Tc-EC-peptide A. Results of in vitro assays showing significant uptake of peptide A compared to 99m Tc-EC control in (a) SUM159, (b) MDA-IBC3, and (c) 13762 breast cancer cell lines. Data is represented as % uptake per mg/protein. Error bars represent standard deviations. Significance is represented by asterisk. * P ≤ 0.001.

Article Snippet: Female Fischer 344 rats (150 ± 25 g, n = 6) (Harlan Sprague-Dawley, Indianapolis, IN) were inoculated subcutaneously with 0.1 mL of a 13762 breast carcinoma cell suspension (10 5 cells/rat of a breast tumor cell line specific to Fischer rats) into the hind legs using 25-gauge needles.

Techniques: In Vitro

Journal: BioMed Research International

Article Title: Patched Targeting Peptides for Imaging and Treatment of Hedgehog Positive Breast Tumors

doi: 10.1155/2014/525680

Figure Lengend Snippet: In vivo uptake of 99m Tc-EC-peptide A in rats bearing 13762 breast carcinoma xenografts. Tumor-to-muscle (T/M) ratios are given for 3 separate rats at multiple timepoints after injection of the radiotracer. Arrows indicate tumor location.

Article Snippet: Female Fischer 344 rats (150 ± 25 g, n = 6) (Harlan Sprague-Dawley, Indianapolis, IN) were inoculated subcutaneously with 0.1 mL of a 13762 breast carcinoma cell suspension (10 5 cells/rat of a breast tumor cell line specific to Fischer rats) into the hind legs using 25-gauge needles.

Techniques: In Vivo, Injection